{"id":446,"date":"2024-01-10T11:52:13","date_gmt":"2024-01-10T11:52:13","guid":{"rendered":"https:\/\/britainwriters.com\/answers\/?p=446"},"modified":"2024-01-10T11:52:14","modified_gmt":"2024-01-10T11:52:14","slug":"course-work-serum-protein-electrophoresis-report","status":"publish","type":"post","link":"https:\/\/britainwriters.com\/answers\/course-work-serum-protein-electrophoresis-report\/","title":{"rendered":"Write a serum protein electrophoresis report"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Course Work &#8211; Serum Protein Electrophoresis Report<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>General Instructions<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Please attend the timetabled practical session<br>Follow the presentation on Learning Zone<br>Familiarise yourself with the background, principle and methodology given in the handbook; Do extra reading to explore more particularly on the background and significance on the technique and its applications<br>Familiarise yourself with the marking criteria for the assessment components<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Background information and Aims of the Session<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">What is Serum?<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">What is Serum Protein Electrophoresis (SPE)?<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Where and when is SPE used?<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Aims of the session<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Understand the difference between serum and plasma<\/li>\n\n\n\n<li>Understand the principle of serum protein electrophoresis<\/li>\n\n\n\n<li>Perform serum protein electrophoresis of the given serum sample (4-5 students per group)<\/li>\n\n\n\n<li>Evaluate and be able to interpret the results<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Materials<\/strong><br>Sample<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Standard serum (Sigma H4522; undiluted)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>For Electrophoresis and staining<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Helena SAS-MX SP kit (Helena laboratories; catalogue number: 100100)<br>Electrophoresis equipment; Tris\/Barbital running buffer; Gel tank; Power supply<br>Acid blue stain; Destain; Methanol to fix the gel<br>Purified water to make up buffers<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The background, aims and materials &amp; methods of serum protein electrophoresis are available in the handbook. After the practical session, you will have your gel image (image-1) containing the protein bands of your standard serum sample. In addition, you will be given a gel image of a serum protein electrophoresis of a patient sample (image-2). You are expected to understand the background, understand the aims, materials &amp; methods and use the published literature and text books to write the \u2018results, discussion and conclusion for the laboratory report&#8217; (for details please read below).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Results<br>Suggested word count &#8211; approximately 200-300 words;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Write up &#8211; In this section you need to present and describe the findings from your gel. Remember to comment on &amp; describe the appearance, migration pattern of different bands of your standard serum sample (image-1).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Figure &#8211; gel image-1 &#8211; Make sure you label all the bands, give a title and add a legend to the figure.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Discussion and conclusion<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Suggested word count &#8211; approximately 700-800 words;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In this section, you will successfully relate the findings from your gel (image-1) elaborate on your reasoning by showing how the principle behind the experiment provides an explanation for the results and also of the additional SPE gel image (image-2) that is provided; Compare and contrast the two images (1 &amp; 2). You should also sufficiently address other possible issues pertinent to this experiment; Evaluate the strengths and limitations of the experiment; Finish this section by writing a conclusion including a summary of the findings and sensible suggestions for possible future experiments.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>References<\/strong><br>List any reference you may have used for your write up. The references should be published literature or textbooks. Do not cite the practical handbook, presentation slides or any web-sites other than NHS websites or other diagnostic clinical laboratory web-sites. Use the Harvard (Cite them right) referencing format, details can<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">You have a word limit of 1000 for this laboratory report. Figure legends and references are excluded from the word count. Use Calibri font size 11 or equivalent.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Course Work Submission, deadline &amp; support<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Submission &amp; deadline<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Component: For the course work, students will write a &#8220;Serum protein electrophoresis report&#8221; (~1000 words; 100 marks)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Course Work &#8211; Serum Protein Electrophoresis Report General Instructions Please attend the timetabled practical sessionFollow the presentation on Learning ZoneFamiliarise yourself with the background, principle and methodology given in the handbook; Do extra reading to explore more particularly on the background and significance on the technique and its applicationsFamiliarise yourself with the marking criteria for [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-446","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.9 - 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