5LMS0001 Bioscience Research Methods Assignment.

LMS0001 Bioscience Research Methods Assignment - University of Hertfordshire, UK

Workshop: Experimental Design for Bradford Microtitre Assay

Aims - The aims of this workshop are:

- To design an assay to measure the amount of protein in a sample using small volumes;

- To initiate the design of an experiment to explore the effect of possible interference by detergents on a protein assay.

Learning outcomes - On completion of this workshop students will:

- know how to make up a set of standards from a stock solution in order to construct a calibration curve;

- understand the considerations and constraints when designing an assay using a microtitre plate;

- know how to design an experiment to explore the effect of detergents on a protein assay.

Experiment 1: Determination of the concentration of protein in a sample of cell lysate

The first part of your task is to design an experiment based on the Bradford assay to determine the total protein content of the cell lysate. You will only have small volumes of sample to work with. You should use the practical booklet and your practical write-up from the Level 4 module Practical and Transferable Skills as a source of information in order to prepare for the practical sessions.