Explain the similarities in the working principle of the colorimeter and spectrophotometer.
Biological and Chemical Instrumentation
GENERAL OBJECTIVES:
1. Understand the principles and instrumentation of spectrophotometer and colorimetry.
2. Know the operation and care of flame photometer and Raman spectrometers.
3. Know the operation and care of Atomic absorption spectrophotometers (AAS).
4. Know the operation and care of the X-ray spectroscope.
5. Know the operation and care of electrolytic conductivity bridge; coulometer titration; PH meter; autotitrator; polarograph.
6. Know the operation and care of radioactive detectors and counters.
7. Understand the operation and care of gas chromatographic equipment, fluorimeter, polarimeter and refractometer.
8. Know the concept of hydrogen ion concentration.
9. Know the various types of electrodes used in measuring ions like fluoride, nitrate, etc.
10. Know use of microscopes.
11. Know the principles of autoradiography.
12. Know the use and maintenance of photomicrographic equipment.
13. Know the use and maintenance of colony counter.
14. Know the use and maintenance of autoclave, centrifuge and incubator.
15. Understand the principle of automation and its significance in chemical analysis.
1.1 State the wave length within the electromagnetic spectrum.
1.2 Distinguish between wavelength of light within the visible region and invisible region.
1.3 Explain the similarities in the working principle of the colorimeter and spectrophotometer.
1.4 Identify the various parts of a colorimeter.
1.5 Explain the functions of the parts in 1.4 above.
1.6 State basic similarities and differences between a colorimeter and spectrophotometer.
1.7 Explain the limitations of colorimeter in microbiological studies.
1.8 Explain the term spectrophotometry.
1.9 List the various sources of light for spectrophotometric determination.
1.10 Describe diffraction grating
1.11 Explain the functions of diffraction grating in spectrophotometry.
1.12 Explain the term interference filter.
1.13 State the function of optical filter in spectrophotometry.
