What absorbance measurement should you get for the blank control?

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Last Updated: 07-Sep-23

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QUESTION ONE

To which bin would you discard your used culture plates? Tick the correct line.

	Sharps
	Clinical waste (yellow/ orange bag)
	Autoclave waste (transparent bag)
	General waste (black bag)

QUESTION TWO

To which bin would you discard your used microscope slides? Tick the correct line.

Sharps

Clinical waste (yellow/ orange bag)

Autoclave waste (transparent bag)

General waste (black bag)

You are required to prepare a series of standards of bovine serum albumin (BSA) for a Bradford assay. You are given a BSA stock (concentration of 1.5 mg/mL) and phosphate buffered saline (PBS) to dilute the samples. The final concentrations of each of the five samples are listed in the table below, which should each have a final volume of 30 ?l.

Calculate the volumes of BSA and PBS required for each sample.

Sample number	Final concentration BSA (mg/ml)	Volume BSA stock (?l)	Volume PBS (?l)
1	0	0	30
2	0.25	5	25
3	0.5	10	20
4	1.0	20	10
5	1.25	25	5

QUESTION NINE

If you were to analyse these samples in a spectrophotometer, please name the blank control solution you would use.