What changes in the dna sequence would have to happen

On the activity Investigating CFTR -

Question 1, you transcribed and translated part of the CFTR gene. Located the 85th amino acid on this activity. What is this amino acid?

Question 2. What changes in the DNA sequence would have to happen if the amino acid was changed to a Glutamic Acid? This mutation is called the G85E. Hypothesize what this might do to the function of this protein.

Question 3. Accept the site usage agreement, and typing G85 in the first variant box. (If you type all of G85E, it will not work.) Select this mutation when it appears and click submit. Switch to healthcare/science view if you are on general view. Does this mutation lead to a disease? How many people have the mutation? Pick Functional Testing. What of mutation is this?

Question 4. Select 3 other mutation of this gene using this website. (Just start putting anything in the variant one box then pick a mutation.) One of your selections must be a different type of mutation than G85E. Categorize each mutation as to of mutation, whether it leads to disease or not and the prevalence of the mutation. A. B. C.